Journal: Molecular Therapy. Nucleic Acids
Article Title: Overexpression of lncRNA EPB41L4A-AS1 Induces Metabolic Reprogramming in Trophoblast Cells and Placenta Tissue of Miscarriage
doi: 10.1016/j.omtn.2019.09.017
Figure Lengend Snippet: The Expression of EPB41L4A-AS1 Increases in Placental Tissue of Early RM (A) Real-time PCR was used to determine the levels of significant differences in long non-coding RNAs in early RM placental trophoblast cells after lncRNA microarray (N = 3). EPB41L4A-AS1 was significantly higher in early RM groups (p < 0.05). (B) EPB41L4A-AS1 transcription levels were increased significantly in some pregnancy pathologies (N = 232) (p < 0.01) compared with normal placental tissue: early RM (N = 62, p < 0.01), IUGR (N = 36, p < 0.05), and PE (N = 144, p < 0.05). These data were collected from the GEO database. (C) The expression level of EPB41L4A-AS1 in our clinical sample was statistically higher in early RM placental tissue (N = 45) compared with the controls (N = 23), as shown by real-time PCR. (D) TIGA1 protein expression was increased dramatically in early RM placental tissue, as shown by western blot examination and quantitative analysis (N = 8) by ImageJ software. (E) Immunohistochemical staining of TIGA1 in early RM placental tissue. Quantitative analysis of TIGA1 intensity (N = 40) was performed using PerkinElmer Vectra 2. Stained TIGA1 was significant higher in the early RM group. Data are represented as means ± SD; *p < 0.05, **p < 0.01, Student’s t test.
Article Snippet: Quantitative analysis of TIGA1 intensity (N = 40) was performed using PerkinElmer Vectra 2.
Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray, Western Blot, Software, Immunohistochemical staining, Staining
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